Rheumatoid Arthritis: Methods and Protocols: 1868 (Methods in Molecular Biology)
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Autor: Shuang Liu
Editura: Springer
Limba: Engleza
Nr. pagini: 268
Coperta: Paperback
Dimensiuni: 17.81 x 1.6 x 25.4 cm
An aparitie: 10 Dec. 2019
Description:
This volume provides a summary of the standard laboratory protocols and methodology commonly used in basic and translational studies in the field of rheumatoid arthritis (RA) treatment. Chapters detail including basic RA models, evaluation of disease activity and immunological status, systemic drug delivery, and new research tools. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Rheumatoid Arthritis: Methods and Protocols aims to be a basic manual for clinical researchers who are just getting started in the field of intervention study.
Table of contents:
Collagen-Induced Arthritis Models
Pages 3-7
Miyoshi, Maya (et al.)
Human Xenograft Model
Pages 9-15
Liu, Shuang
Long-Term Constant Subcutaneous Drug Administration
Pages 17-22
Liu, Shuang (et al.)
Clinical Scoring of Disease Activity in Animal Models
Pages 23-28
Miyoshi, Maya (et al.)
Histological Analysis of Arthritic Joints
Pages 29-39
Kiyoi, Takeshi
Preparation of Joint Extracts
Pages 41-45
Liu, Shuang (et al.)
Production of Immunizing Antigen Proteoliposome Using Cell-Free Protein Synthesis System
Pages 49-67
Zhou, Wei (et al.)
Reconstruction of Protein/Liposome Complex
Pages 69-77
Suzuki, Yasuyuki
Production of Neutralizing Antibody
Pages 79-92
Takemasa, Erika (et al.)
Autoantibody Profiling Using Human Autoantigen Protein Array and AlphaScreen
Pages 93-112
Takeda, Hiroyuki
Generation of Specific Aptamers
Pages 113-121
Liu, Shuang (et al.)
Production of Lentiviral Particles
Pages 123-128
Liu, Shuang
RNA Interference Ex Vivo
Pages 129-135
Liu, Shuang
Lentiviral-Mediated Systemic RNA Interference In Vivo
Pages 137-144
Liu, Shuang
Mesenchymal Stem Cell Engineering
Pages 145-150
Liu, Shuang
Screening of Ca2+ Influx in Lymphocytes
Pages 153-159
Takemasa, Erika (et al.)
Single-Cell Ca2+ Imaging
Pages 161-168
Liu, Shuang
Electrophysiological Methods to Measure Ca2+ Current
Pages 169-176
Liu, Shuang (et al.)
The Functional Assessment of T cells
Pages 177-199
Maruyama, Saho
Release of Antibodies and Cytokines from B Cells
Pages 201-207
Liu, Shuang
Evaluation of Autoreactive Responses
Pages 209-213
Liu, Shuang
Bone Resorption Activity in Mature Osteoclasts
Pages 215-222
Kiyoi, Takeshi
Animal Models of Vasculitis
Pages 223-232
Mogi, M. (et al.)
Design an Intervention Study
Pages 235-241
Hasegawa, Hitoshi
Assessment of Disease Activity, Structural Damage, and Function in Rheumatoid Arthritis
Pages 243-250
Hasegawa, Hitoshi
Correction to : Production of Lentiviral Particles
Pages E1-E1
Liu, Shuang
| An aparitie | 10 Dec. 2019 |
| Autor | Shuang Liu |
| Dimensiuni | 17.81 x 1.6 x 25.4 cm |
| Editura | Springer |
| Format | Paperback |
| ISBN | 9781493993932 |
| Limba | Engleza |
| Nr pag | 268 |
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