Immunohistochemistry: A Technical Guide to Current Practices

This book will enable practitioners to understand the many complex intricacies of immunohistochemistry (IHC) and make best use of this powerful analytical tool. Providing a thorough grounding in the fundamentals of immunohistochemistry, the book includes several chapters on robotics and automation technology, giving key information on the design of machines and tips to maximise workflow efficiencies. The relationship between IHC and molecular pathology is explained clearly, demonstrating the increasing impact on personalized medicine and targeted therapies for cancer patients. The staining protocol is deconstructed, allowing the reader to adapt it for a variety of diagnostic and research applications. Written by experts at the forefront of hospital immunohistochemistry, there is a strong emphasis on practical guidance on a range of techniques as well as troubleshooting of common problems driven by the authors' experiences. Extensively illustrated with high-quality colour images, this is an invaluable resource to all pathology practitioners utilising the technique.

Table of Contents:

1. Immunohistochemistry fundamentals Enisa Hasic; 2. Immunohistochemistry quality assurance and quality control Bronwyn Christiansen & Hazel Chambers; 3. Automation and robotics with the Leica Bond III Anthony F. Henwood; 4. Automation and robotics with the Roche ventana benchmark ULTRA; 5. Automation and robotics with the Agilent dako omnis Andrew Dinan; 6. Immunohistochemistry for research applications Trung Nguyen; 7. Troubleshooting immunohistochemistry David Gan; 8. Current status of immunohistochemistry Trung Nguyen; 9. Immunohistochemistry for future applications Candice J Clarke.