Immunology: Overview and Laboratory Manual

Section I of this book provides an overview of the immune system and immunity, and includes review questions, problem sets, case studies, inquiry-based questions, and more to provide students with a strong foundation in the field. New chapters on trained immunity, oral immunity, mucosal immunity, chimeric antigen receptor (CAR) T cells, and immune checkpoint inhibitors have been added to the second edition. Section II consists of thirty-seven lab exercises focused on key concepts in immunology, such as antibody production, cell separation, cell function, immunoassays, Th1/Th2 cytokine detection, chromatin immunoprecipitation (ChIP), nitric oxide detection, cell and tissue culture methods, and cell and molecular biology techniques. Appendices include safety information, suggested links and readings, and standard discipline processes, protocols, and instructions.